3,144reference species
218query proteins
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How phylogenetic profiling works

Large‑scale
phylogenetic
profiling

Turn a BLAST search into interactive species trees, domain clusters and heatmaps — explore presence/absence patterns across whole proteomes and read gene function straight from the data.

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upload a BLAST file & explore
phylogenetic profiling BLAST Neighbour-Joining Markov clustering heatmaps bioinformatics
The toolkit

Five tools, one pipeline

BLAST Analysis

Build the species × domain correlation or feature matrix from a BLAST file.

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Tree builder

Neighbour-Joining species tree from Jaccard distances between profiles.

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Tree viewer

Collapsible radial tree — colour by genus, search a species, zoom.

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All-vs-all

Cluster domains (MCL) into an interactive network linked to a co-cluster heatmap.

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Heatmaps

Feature/correlation heatmaps with ordering, log scale, zoom and cell details.

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How to use

A plain-language guide for biologists and programmers alike.

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About

Built at the BCCB Group, AUTH

PhyloFlask is a software framework for large-scale phylogenetic profile visualization by A. Michailidis, V. S. Papagrigoriou & C. A. Ouzounis (Biological Computation & Computational Biology Group, Artificial Intelligence & Information Analysis Laboratory, School of Informatics, Aristotle University of Thessaloniki).

It analyses presence/absence patterns of protein domains across thousands of reference proteomes to enable rapid, scalable visual inference of gene function and evolutionary relationships — facilitating hypothesis generation for experimental validation.

Read the poster

Reference: Cohen BA, Mitra RD, Hughes JD & Church GM (2000). A computational analysis of whole-genome expression data reveals chromosomal domains of gene expression. Nature Genetics 26(2), 183–186. doi:10.1038/79896